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Effect of inclusion of black soldier fly larvae (Hermetia illucens) meal in diets on growth performance and meat quality of growing quail

Nguyen Hai Quan, Vo Thi Minh Tam, Le Duc Thao and Le Duc Ngoan

Faculty of Animal Sciences and Vet. Medicine University of Agriculture and Forestry, Hue University 102 Phung Hung, Hue City, Viet Nam
nhquan@hueuni.edu.vn

Abstract

This study aimed to evaluate the effect of inclusion levels of Black Soldier Fly Larvae (BSFL) meal in diets on growth performance, carcass traits and proximate composition of quail meat. Total 480 quails of 17.03 ± 0.42 g (Mean ± Std) randomly allocated into 4 treatmentary diets, including CTL (control diet), B9, B18 and B27, in which, 9, 18 and 27% of BSFL meal (as DM) were used, and 3 replicates. Feed intake, average daily gain were recorded and the final weight, quails meat component quantity and chemical composition of meat were measured at the end of the experiment. Results on growth performance show that inclusion of BSFL meal in diets improved average daily gains of broiler quails, daily feed intake but not feed conversion ratios. Carcass dressing and percentage of breast and thighs meat, crude protein and ether extract contents did not affect by inclusion of BSFL meal but increasing inclusion of BSFL meal (9-27% as DM diet) raised dry matter content of quail meat.

Keywords: carcass characteristics, breasts and thighs meat, soybean meal


Introduction

Recently, quail production has been successfully developing in many countries, since quail has a number of productive and economic advantages over other bird species (Minvielle 2009). The genetic potential productivity of Japanese quail is quite high as their egg production reaches 310 eggs per year with an average weight of 12.5 g (Vali 2008). Quail meat, which is superior in nutritional and gustatory quality to meat of all types of farm poultry, is no less valuable dietary and medicinal food product than quail eggs. Tender, juicy and flavorful quail meat is a delicacy (Santhi and Kalaikannan 2017).

In Viet Nam, quail production has been rapidly developing. According to GSO (2021), quail population annually increased 11% and meat production of 37.9%/year during 2015-2020. In 2020, quail population was 31,409 thousand heads and meat production is 8649.9 tones. Quail required higher protein in diet (21-23%) than chicken (15-20%). Fishmeal and soybean meal are main protein sources in quail diets and their prices are increasing, therefore we have to look at an alternative protein sources.

The black soldier fly (Hermetia illucens) has been studied in recent years and its larvae can be used as an unconventional feed resource for animals and aquaculture and can replace fishmeal and soybean meal (Spanghers et al 2017; Moula et al 2018; Lan et al 2022a). Black soldier fly larvae (BSFL) have a high crude protein (37-63% as DM) and crude fat (7-39%), and these values ​​vary widely depending on substractes for feeding the larvae (Ngoan et al 2021). Black soldier fly larvae have been used in diets of broiler and laying quails (Cullere et al 2016a,b; 2017; Mat el al 2021; Mulyono et al 2021). Cullere et al (2016b) reported that inclusion of 10 and 15% in diets did not affected growing performance and breast weight and yiled of broiler quails. The authors concluded that BSFL meal can partlly replace conventional soyabean meal and soyabean oil in diets for growing broiler quails, thus confirming to be a promising insect protein source for the feed industry.

In Viet Nam, BSFL have been recently using for chicken (Ta Trung Nghia 2020) and fish (Hoa and Dung 2016; Lan et al 2022a). Ta Trung Nghia (2020) reported that including BSFL in diets improved survivial rates, growth performance and feed conversion ratio of local Mia chicken and economic benefit. However, the use of BSFL for feeding quail has not been publishing recently yet. This study therefore, aimed at evaluating the effects of including BSFL meal levels in diets on growth performance and meat quality of growing quails.


Materials and methods

The experiment was carried out at Centre for Animal Practice and Training, Faculty of Animal Sciences and Veterinary Medicine, University of Agriculture and Forestry, Hue University during March- June 2022.

Animal and diet preparation

uails were purchased in the Quail farm at Thuy Duong commune, Huong Thuy District, Thua Thien Hue province. They were nursed for one weeks before running the experiment. During the experiment, animals were fed three times, at 7.00; 13.00 and 17.00 and water was supplied enough.

Larvae meal preparation: BSFL were fed by tofu by-products and collected at day 7th after rearing). Larvae meal preparation was followed Lan et al (2022b). Larvae were washed with water several times to remove all impurities, boiled at 60oC water for 1 minutes, took out and dried at 55°C for 24 hrs and milled into full - fat BSFL meal.

Diet preparation: All ingredients including BSFL meal were carefully mixed according to their proportion in the diet, dried at 45°C for 24hrs and stored in plastic bags at room temperature.

Experimental design

Total 480 quails of 17.03 ± 0.42 g (Mean ± Std) randomly allocated into 4 dietary treatments, including CTL (control diet), B9, B18 and B27 equivalent to 9%, 18% and 27% of BSFL meal. Each treatment was repeated 3 times Diets were formulated to demand quail’s nutrient requirement (NRC 1994). Chemical compositions of ingredients and diets indicate in Tables 1 and 2.

The feeding experiment was lasted for 30 days.

Table 1. Chemical composition of feeds (%)

Ingredients

DM

CP

EE

Ash

Cassava root meal

88.3

2.59

3.23

1.60

Maize meal

86.0

8.26

3.64

0.60

Oil extracted bran

88.8

15.9

0.50

15.3

Soybean meal

88.4

50.5

2.29

6.44

Fish meal

86.8

51.0

4.75

16.6

BSFL#

86.7

51.3

26.7

8.90

#BSFL: Black soldier fly larvae



Table 2. Ingredient proportion and chemical composition of diets

Treatment

CTL

B9

B18

B27

Ingredient (%)

Maize meal

49.5

50.2

50.2

50.2

Oil extracted bran

8.48

8.61

9.30

5.00

Cassava root meal

3.99

3.00

2.19

5.00

Soybean meal

36.0

27.0

18.0

9.00

Fish meal

1.39

1.59

1.71

3.20

BSFL meal

-

9.00

18.0

27.0

Salt

0.30

0.30

0.30

0.30

Premix mineral-vitamin

0.30

0.30

0.30

0.30

Chemical composition (%)

DM

89.1

89.1

89.0

87.9

CP

25.0

25.7

25.3

25.2

EE

4.43

6.47

9.06

9.73

Ash

3.64

4.59

5.27

5.99

Measurements

Growth performance: All quails were weighted weekly in the morning and then daily weight gain (ADG) was calculated. Daily feed refusals were weighted and daily feed intake was calculated accordingly, and feed conversion ratio (FCR) was defined as following:

Carcass characteristics: At the end of the experiment, 6 quails (3 males and 3 females) per treatment were slaughtered for carcass characteristics and meat quality. Quails were slaughtered following the commercial methods as normal practice. The weights at slaughter, carcass, and breasts and thighs were measured at slaughter time. Following characters were calculated:


Meat quality: Samples of breasts and thighs were collected for chemical analysis: DM, CP, EE and total ash following AOAC (1990) procedure at the Lab of Faculty of Animal Sciences and Veterinary Medicine.

Data analysis

The data were statistically processed by analysis of variance (ANOVA) using General Linear Model in Minitab 16.2.0 (2010). The difference between the mean values was determined by the Tukey method at a confidence level of 95%. Statistical model:

Yij = µ + αi + ɛij

Where: µ is the population mean; αi is the effect of levels of larvae meal (i= 1-4); eijk is the random effect


Results and discussion

Growth performance

Data on live weight, daily weight gain, feed intake and feed conversion ratio are presented in Table 3, Figure 1, Figure 2 and Figure 3.

Figure 1. Correlation between BSFL meal levels and live weight Figure 2. Correlation between BSFL meal levels and live weight

Figure 3. Correlation between BSFL meal levels and FCR


Table 3. Growth performance of quails fed diets containing black soldier larvae meal

Item

Dietary treatment

SEM

p

CTL

B9

B18

B27

Initial weight (g)

16.8

16.8

17.3

17.2

0.24

0.40

Final weight (g)

121.8a

138.9b

138.0b

140.4b

4.09

0.04

ADG (g)

3.50a

4.07b

4.02b

4.11b

0.13

0.037

Feed intake (g/day)

13.32d

15.93c

17.42a

16.35b

0.096

<0.001

FCR

3.82

3.93

4.33

3.99

0.13

0.099

a,b,c : Means in the same row for each parameter with different superscripts are significantly different (p <0.05)

Growth performance is an important criterion in poultry production in general and in quail production in particular. This is an indicator that determines the meat production capacity of quails and has great economic significance in quail production. The growth performance of quail was also evaluated through the average daily gain (ADG). Data in Table 3 show that ADG is higher in diets including BSFL meal than that in CTL (p<0.05). Values of ADG in B9, B18 and B27 range 4.04-4.11 g vs 3.5 g in CTL. Feed intake is an important indicator in poultry production and is directly affected by nutrient’s concentration in the diet, and other factors. Through this indicator, it is possible to assess the health status of poultry flocks, the quality of feed and the level of feeding and care. Daily feed intake reflects also palatability and feed suitability for quail. Data in Table 3 indicate that daily feed intake is increasing when increasing levels of BSFL meal in diets. Daily feed intake is highest in B18, then B27, and lowest in CTL (p<0.05). Feed conversion ratio (FCR) represents the efficiency of feed utilisation, which is an important indicator in quail production. One of many factors affect in FCR is characteristics of the diet (nutrition value, feed processing technology, feed quality). Results in Table 3 show that FCR values intend to increasing levels of BSFL however are not significantly defferent among treatments (p>0.05), range 3.82-4.33. The correlation coefficents between live weight, ADG, FCR and increasing BSFL rates in diets are presented in Figures 1, 2 and 3, respectively.

Cullere et al (2016b) reported that inclusion of 10 and 15% of BSFL meal in soyabean meal-based diets did not affect ADG, feed intake and FCR of quails. ADG ranged 8.24-8.40 g/day; feed intake ranged 23.3-24.4 g/day and FCR 2.83-2.90. Mat et al (2021) found that, inclusion of 20 and 25% of BSFL meal in broiler quail diets improved ADG, daily feed intake and FCR. These authors indicated that ADG of quails fed 20 and 25% BSFL meal-based diets (3.3-3,4 g/day) were higher than that in control and 15% BSFL meal-based diets (2.1-2.8 g/day). Mulyono et al (2021) studied on replacing fishmeal by BSFL meal plus Trichoderma in laying quails and found that daily feed intake reduced at level of 100% replacement, but FCR maintained.

The results of this study showed that inclusion of BSFL meal 9-27% as DM diet improved average daily gains of broiler quails, daily feed intake but not feed conversion ratios.

Carcass traits

Data on carcass characters of quail meat present in Table 4. Absolute value of carcass, thighs plus breasts weights are different among treatments due to different slaughter weight. However, carcass dressing and percentage of breasts plus thighs are not significant among them (p>0.05). In general, including BSFL meal in diets does not affect carcass characteristics of quail meat.

Table 4. Carcass characteristics of quails fed diets containing black soldier larvae meal

Items

Treatment

SEM

p

CTL

B9

B18

B27

Slaughter weight (g)

125.0a

151.2b

143.5b

143.0b

2.82

0.001

Carcass weight (g)

90.9a

108.5b

97.7ac

101.3bc

2.97

0.018

Carcass percentage (%)

72.72

71.78

68.08

70.78

1.69

0.287

Weight of thighs and breasts (g)

35.35a

44.07b

40.39c

36.25a

1.10

0.002

Percentage of thighs and breasts (%)

38.89

40.62

41.34

35.87

1.45

0.101

a,b,c : Means in the same row for each parameter with different superscripts are significantly different (p <0.05)

Cullere et al (2016b) found that carcass dressing and percentage of breast meat yield were not significant different among treatmentary diets of broiler quails fed the control diet and diets containing 10 and 15% BSFL as DM. These authors reported that carcass dressing ranged 64.1-64.7% and breast percentages of 30.7-30.8%. In addition, Cullere et al (2016b) reported also that inclusion of 10 and 15% of BSFL meal reduced meat pH values from 5.76 (control diet) to 5.67-5.68 (diets contained BSFL meal).

The results of this study show that carcass dressing and percentage of breast and thighs meat did not affect by inclusion of BSFL meal in diets of growing quails.

Chemical composition of meat

Data on chemical composition of meat indicate that DM and total ash contents are affected by BFSL inclusion (p<0.05) but not CP and EE (p>0.05). DM content is higher in B9 and B18 than that in CTL and B27 (p<0.05). Content of total ash is highest in B9 and CTL and lowest in B27. Differences in DM and ash contents do not reflect the diet effects on chemical composition of quail meat.

Table 5. Chemical composition of quail meat fed diets containing black soldier larvae meal

Item

Dietary treatment

SEM

p

CTL

B9

B18

B27

DM

26.13a

26.61b

26.66b

25.98a

0.10

0.003

CP

84.07

84.70

82.12

81.82

0.74

0.063

EE

9.35

10.11

9.43

10.21

0.58

0.631

Ash

8.74bac

9.25a

8.04b

7.76bd

0.24

0.009

a,b,c : Means in the same row for each parameter with different superscripts are significantly different (p <0.05)

Cullere et al (2016a) reported that inclusion of 10 and 15% BSFL meal in diets did not affect proximate composition and cholesterol concentration of breast meat. However, with increasing BSFL meal, the total saturated fatty acid and total monounsaturated fatty acid proportions raised to the detriment of the polyunsaturated fatty acid fraction thus lowering the healthiness of the breast meat.

The results of recent study show that increasing inclusion of BSFL meal (9-27% as DM diet) did not affect crude protein and ether extract contents but raised dry matter content, and fatty acid profile of meat did not analyze in the study.


Conclusion


Acknowledgement

The authors also acknowledge the partial support of the Strong Research Group Program of Hue University (Code: 06/HD-DHH).


References

AOAC 1990 Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA: AOAC International.

Cullere M, Tasoniero G, Giaccone V, Acuti G, Marangon A and Dalle Zotte A 2016a Black soldier fly as dietary protein source for broiler quails: Meat proximate composition, fatty acid and amino acid profile, oxidative status and sensory traits. Animal 12(3): 640-647. DOI: 10.1017/S1751731117001860

Cullere M, Tasoniero G, Giaccone V, Miotti-Scapin R, Claeys E, De Smet S and Dalle Zotte A 2016b Black soldier fly as dietary protein source for broiler quails: Apparent digestibility, excreta microbial load, feed choice, performance, carcass and meat traits. Animal 10(12): 1923-1930. DOI: 10.1017/S1751731116001270

GSO 2021 General Statistic Office. Statistical Year Book. At https://www.gso.gov.vn/nong-lam-nghiep-va-thuy-san/

Hoa N P and Dung N V 2016 The use of black soldier fly larvae (Hermetia illucens) in diets for snakehead fish (Chanamicropeltes). Journal of Vietnam Agricultural Science, 14(4):590 – 597.

Lan P T P, Ngoan L D, Quan N H and Tram N D Q 2022a Effects of harvesting time on yield, chemical composition of black soldier fly (Hermetia illucens) larvae and replacement of trash fish for feeding seabass (Lates calcarifer Bloch, 1790) rearing in fresh and brackish water. Liv. Res. for Rural Devel., 34(1):3; at http://www.lrrd.org/lrrd34/1/3403ndqtr.html

Lan P T P, Ngoan L D, Tram N D Q 2022b Effect of replacement of fishmeal-based diet by full-fat or defatted black soldier fly larvae (Hermetia illucens) meal in diets on performance of Asian seabass (Lates calcarifer) juvenile in fresh and brackish water.Livestock Research for Rural Development (0121-3784). 34(11):102; https://lrrd.org/lrrd34/10/3484nguy.html.

Mat K, Mohamad N A, Rusli N D, Rahman M M, Harun H C, Al-Amsyar S M and Mahmud M 2021 Preliminary study on the effect of feeding Black Soldier Fly Larvae (BSFL) on growth and laying performance of Japanese quail (Cortunix japonica). Int. J. of Agric. Technology 17(3): 977-986.

Minvielle F 2009 What are quail good for in a chicken-focused world? World's Poultry Science Journal, 65(4), 601—608. doi: 10.1017/S0043933909000415.

Moula N, Scippo M, Douny C, Degand G, Dawans E, Cabaraux J, Hornick J, Medigo R C, Leroy P, Francis F, Detilleux J 2018 Performances of local poultry breed fed black soldier fly larvae reared on horse manure. Animal Nutrition, 4: 73-78.

Mulyono M, Widiyanto W, Mangisah I, Krismiyanto L, Yunianto V D, Ismadi B, Sukamto B, Wahyono F and Suthama N 2021 The substitution of fish meal with larvae of Hermetia illucens supplemented with Tricoderma sp on quail’s nutritional utility and egg production. Livestock Research For Rural Development 33(3):37; at: http://www.lrrd.org/lrrd33/3/qmuly3337.html

Ngoan L D, Quan N H, Lan P T P and Tram N D Q 2021 Using black soldier fly larvae (Hermetia illucens) as animal and aquaculture feeds. Vietnam Journal Agriculture and Rural Development (2), 141-150.

NRC 1994 Nutrient Requirements of Poultry: Ninth Revised Edition. National Academies Press. Washington D.C

Ta Trung Nghia 2020 The use of black soldier fly larvae for feeding chicken in Quang Binh Province. Quang Binh Scientific and Technology News; No2/2020: 60-62; at: https://skhcn.quangbinh.gov.vn/3cms/upload/khcn/File/BanTinKHCN/2020/so2/So2_2020_20.pdf

Santhi D & Kalaikannan A 2017 Japanese quail (Coturnix coturnix japonica) meat: Characteristics and value addition. World's Poultry Science Journal,73(2), 337—344. doi: 10.1017/S004393391700006X

Spranghers T, Ottoboni M, Klootwijk C, Ovyn A, Deboosere S, Meulenaer B D, Michiels J, Eeckhout M, Clercq P D, Smet S D 2017. Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture, 97 (8), 2594-2600.

Vali N 2008 The Japanese quail: A review. International Journal of Poultry Science, 7(9), 925—931. doi: 10.3923/ijps.2008.925.931.