Effect of water spinach and fresh cassava leaves on intake, digestibility and N retention in growing pigs

Chhay Ty and T R Preston^*

Center for Livestock and Agriculture Development (CelAgrid-UTA Cambodia)
PO box 2423, PhnomPenh, Cambodia
chhayty@utafoundation.org
^*University of Tropical Agriculture (UTA-Colombia)
AA #48, Socorro, Santander, Colombia
regpreston@utafoundation.org

Abstract

The experiment was conducted in the Center for Livestock and Agriculture Development (CelAgrid-UTA Cambodia). Three Local x Large White or Durok male pigs weighing on average 15.1 kg were allotted at random to three diets to study the effect on digestibility and N retention of water spinach and fresh cassava leaves, or a mixture of the two, as supplements to broken rice. The design was a 3x3 Latin square with periods of 14 days. Foliage was harvested daily from a late maturing cassava variety (12-15 months) beginning 5 months after planting. Fresh cassava leaves and fresh water spinach were chopped into small pieces and fed ad libitum. Broken rice was restricted at a level of 2% of body weight (DM basis).

The HCN content of the fresh cassava leaves was 508 mg/kg DM resulting in an intake of  9.4 mg HCN/kg live weight. During the entire trial the pigs were in a positive body weight balance and there were no symptoms of toxicity from feeding fresh cassava leaves. Total DM intake, digestibility of DM, OM, N and crude fiber, and N retention, were higher with fresh water spinach and the mixture between fresh water spinach and cassava leaves, as compared with fresh cassava leaves alone.

It is concluded that: fresh cassava leaves can be fed at 41% of the diet DM with no apparent signs of toxicity; fresh water spinach is more palatable than cassava leaves as reflected in higher total DM intake and the proportion of the diet (47%) provided by the leaves.

Key words: broken rice, digestibility, fresh cassava leaves, N retention, pigs, water spinach

Introduction

Monogastric animals play an important role in agricultural activities in rural areas in Cambodia.  On average, a farmer raises 2 to 5 heads of pigs (local or crossbred). The major problem in pig production is the lack of protein because protein-rich feed resources are scarce and, if available, the prices are often prohibitive. There is a need to identify feeds, which can compensate for these deficiencies.

Water spinach (Ipomoea aquatica) is a water plant. It is cultivated for human food and used for pigs and other animals in Cambodia. It does not appear to contain anti-nutritional compounds and has been used successfully for growing pigs as the only source of supplementary protein in a diet based on broken rice (Ly 2002). In that research there was a significant response in growth rate and feed conversion to supplementary DL-methionine. Prak Kea et al (2003) reported a linear increase in growth rates in pigs fed water spinach, palm oil and broken rice when up to 6% fish meal replaced equivalent amounts of water spinach, which they attributed to an improved amino acid balance, especially in terms of the sulphur-rich amino acids.

Cassava (Manihot esculenta Crantz) is a widely grown crop in many tropical counties (Calpe 1992). Traditionally it is cultivated for root production, but recently attention has focused on managing it as a semi-perennial forage with repeated harvesting at 2-month intervals (Preston 2001). In this system,  when the cassava is fertilized with high levels of organic manure, annual protein yields can reach 3 to 4 tonnes/ha.

Nutritional experiments with pigs using cassava leaves have concentrated on processing the leaves by ensiling (Ly et al 2001), as this is a safe way of reducing the concentration of the precursors of HCN, which are the toxic elements in fresh cassava leaves. Sun drying is even more effective than ensiling as a means of reducing the risk from HCN toxicity (Oke 1994). However, this is almost impossible to do in the rainy season and is also time consuming. Ensiling the leaves can be done all-year round but requires infrastructure and is also a laborious procedure. If cassava leaves could be fed fresh to pigs this would simplify the management considerably.

The protein in cassava leaves has been reported to be rich in lysine but limiting in methionine (Eggum 1970).  Water spinach also appears to be limiting in methionine in view of the growth response when synthetic DL-methionine was added to a diet in which water spinach provided most of the protein (Ly 2002). Mixing two sources of leaves as sources of protein may be beneficial in view of possible complementary effects of the two arrays of amino acids.

The aim of the present experiment was to study the effect of water spinach and fresh cassava leaves, fed alone or mixed together, on intake, digestibility and N retention of growing pigs fed a low-protein, basal diet of broken rice.

Materials and Methods

Location and climate

The experiment was carried out from 5 September to 11 October 2004 at the Center for Livestock and Agriculture Development (CelAgrid-UTA Cambodia), located in Kandal village, Rolous Commune, Kandal steung district, Kandal province about 25km from Phnom Penh City, Cambodia . During the trial, the temperature was about 27 ^ºC in the morning, 31 ^ºC in the middle of the day and 30 ^ºC in the evening.

Experimental feeds

The daily dry matter (DM) allowance was calculated at 4% of the body weight. The broken rice was restricted to 2% (DM basis) of live weight, so as to stimulate the pigs to eat the forages. DL-methionine was included at the level of 0.3% (in DM). Fresh cassava leaves were harvested everyday in CelAgrid-UTA Cambodia, beginning at 5 months after planting the cassava.  Stems and petioles were removed from the leaves and then the leaves were chopped into small pieces and offered immediately. Water spinach was purchased from the farmers near the center and was chopped into small pieces and offered immediately at the same time as the fresh cassava leaves. The broken rice was purchased from a local rice mill near CelAgrid. Data on composition of the feeds are in Table 1.

Treatments and design

There were three treatments:

• FC: Fresh cassava leaves

• WS: Water spinach

• WSFC: Mixture between fresh water spinach and fresh cassava leaves (50:50 DM basis)

The experiment was done according to a 3*3 Latin Square arrangement of the 3 dietary treatments with 3 crossbred (Local x Large White or Duroc)  young male pigs weighing on average 15.1 kg. The animals were housed in metabolism cages during the whole trial (36days). The metabolism cages (80 x 80cm) were built to allow the quantitative collection of faeces and urine (Photo 1). The characteristics of the cages have been described elsewhere (Chhayty et al 2003ab). The metabolism cages were installed in an open stable. Each experimental period consisted of 12 days; 7 days to adapt to the diets followed by another 5 days for collection of faeces, urine and feed refusals.

Photo 1: The metabolism cage used in the experiment

The pigs were fed thrice daily with equal rations at 8:00 am, 12:00 am and 3:00 pm. The fresh cassava leaves, fresh water spinach or mixture were mixed with the other components of the ration (broken rice, premix and methionine). The mixture of leaves was in the ratio 35 of cassava leaves and 65 of water spinach (fresh basis). Water was permanently supplied through drinking nipples. The animals were weighed at the beginning of the trial and every 12 days.

Data collection

Feed refusals and faeces were collected every day and were kept frozen in plastic bags until analysis. A representative sample was obtained from every treatment. At the end of each period, feed refusals and faeces were mixed thoroughly by hand and then homogenized in a coffee grinder, prior to taking representative samples that were analyzed for DM, N, crude fibre, HCN and ash. Urine was collected in a plastic bucket to which sulphuric acid was added to maintain the pH below 4.0. The volume of urine was measured every day and at the end of each period a sample was analyzed for N.

Chemical analyses

Chemical analyses of the feed ingredients and faeces were undertaken following the methods of AOAC (1990) for ash, N and crude fibre. The DM content was determined using the microwave method of Undersander et al. (1993). Fresh faeces were analyzed for pH with a glass electrode. The N content of urine and HCN in fresh cassava leaves were determined by the AOAC (1990) procedures.

Statistical analyses

The data were subjected to analysis of variance according to the general linear model of the Minitab software (Minitab release 13.31, 2000). Sources of variation were pigs, periods, treatments and error.
 

Results

During the whole trial the pigs gained weight and there were no symptom of intoxication due to the fresh cassava leaves. The urine from the pigs fed fresh cassava leaves had a darker red colour compared with urine from pigs fed water spinach (Photo 2).

Feed intake

All the broken rice was consumed. Total voluntary feed intake was higher on the two diets containing water spinach compared with only cassava leaves (Table 3). The proportion of the total diet in the form of foliage was highest with water spinach alone and lowest when cassava leaves alone were the source of foliage.

Figure 1: Intake of crude protein from dietary components	Figure 2: Proportion of dietary crude protein from dietary components

Faecal characteristics in pigs fed fresh cassava leaves, fresh water spinach

The dry matter content of the faeces was highest when the pigs were fed fresh cassava leaves and lowest for those fed water spinach (Table 5). Faecal pH tended to be higher  for pigs fed only the water spinach as supplement.

Nutrient digestibility

Digestibility of DM, OM, N and crude fiber decreased as cassava leaves replaced the water spinach (Table 6; Figure 3).

N balance

Daily N retention was lowest but the proportion of digested N that was retained was highest when the protein supplement was only cassava leaves (Table 7).

Received 11 December 2004; Accepted 10 January 2005; Published 1 February 2005

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