Livestock Research for Rural Development 14 (5) 2002 | Citation of this paper |
The direct method of digestibility indices estimation was compared to the indirect technique by use of the acid insoluble acid as natural inner marker in two experiments designed in a factorial arrangement 2x2 with four replications per treatment. In experiment 1, eight Mong Cai pigs of 12.5 to 15.5 kg live weight were utilized. Experiment 2 was conducted with eight Large White pigs of 15.0 and 25.0 kg live weight. In both experiments the diets were formulated with wheat bran and fish meal to contain approximately 18 % crude protein and were offered at the rate of 4.5 kg DM per 100 kg live weight.
There was no influence of live weight on digestibility values. In in Mong
Cai pigs the indirect method of estimation of digestibility of
nutrients appeared to predict slightly
higher values than the direct method. This effect was significant only for DM and NDF
digestibility. There was no significant influence of live weight or method of
determination on any of the measurements conducted in Large White pigs.
It is suggested that the indirect method of digestibility
determination by using acid insoluble ash as reference substance can be used for the
nutritional evaluation of diets given to young pigs.
Key words: Mong Cai, Large White, pigs, acid insoluble ash, digestibility
The use of indirect
methods for estimating total tract digestibility of nutrients in farm animals is not a new
idea (Schurch et al 1950; Gregory and Dickerson 1952; Kimura
and Miller 1957), and in the case of conventional feeds, the acid insoluble ash technique
has been used extensively since the very early first reports made in
The aim of this
investigation was to determine nutritional values of diets for local pigs in the tropical
environment of
The direct
method of determining digestibility indices was compared to the indirect technique, using
the acid insoluble ash as a natural, inert marker in two experiments with Mong Cai and
Large White castrated male pigs.
Eight Mong Cai castrate male pigs of 12.5 to 15.5 kg live weight were used according to a factorial arrangement in which the main treatments were:
Live weight:
12.5 vs 15.5 kg mean live weight
Digestibility method:
Direct (total collection) vs Indirect (acid insoluble ash)
The diet
consisted of wheat bran 69.9, maize bran 19.9, fish meal 10.1
and vitamins and minerals 0.1% (DM basis). Composition of the diets was: DM 87.5% and (in
dry basis) organic matter 90.9, NDF 52.1 and N 2.91 %.
The design
was the same as in Experiment 1, but Large White castrate male pigs were used and the mean
live weights were: 15.0 and 25.0 kg. were employed in this trial. The diet consisted of
wheat bran 89.9, sun-dried whole fresh water fish 10.0 and vitamins and minerals 0.1% (DM
basis). The chemical characteristics of the diet were DM 88.2%, and (in dry basis),
organic matter 94.2, NDF 45.8 and N 3.06%.
During the
entire experimental period the animals were housed in metabolism cages built with rattan
and local materials (see Chiev Phiny and Rodriguez 2001).
Total collection of feed refusals, faeces and urine were made
during five days following five days of adaptation to the diets. The feed was given at the
rate of 45 g DM/kg live weight and was offered twice daily in equal rations. Water was
freely available. Feed refusals were collected and recorded. Other details concerning the
experimental procedure relating to collection of materials and
All feed and faecal samples were subjected to proximate analysis according to the methods of AOAC (1990). Dry matter content in every sample was estimated by microwave radiation until constant weight (Undersander et al 1993). NDF analyses were conducted as outlined by Van Soest et al (1991). The acid insoluble ash concentration in faeces was determined by treating the ash with a 2N HCl solution (Van Keulen and Young 1977). In addition, pH values were estimated in fresh aliquots of faeces with the aid of a glass electrode.
Data were subjected to the analysis of variance technique (Steel and Torrie 1980). The general linear model included in the Minitab statistical program (Ryan et al 1985) was used in all cases.
The voluntary feed intake was 34.8 and 38.8 g DM/kg live weight in
the Mong Cai
pigs of 12.5 and 15.5 kg mean live weight, respectively. Although few refusals were
recorded during the conduct of the trial, it was considered that the feeding level was
close to ad libitum. Average environmental temperature
(January 2001) was 35 ± 2.5ºC at
Table
1. Comparison of methods of determination of digestibility indices in young Mong Cai pigs |
||||||
Method of estimation |
Live weight, kg |
|||||
Direct |
Indirect |
SEM |
12.5 |
15.5 |
SEM |
|
Digestibility,
% |
||||||
DM |
81.4 |
83.9 |
1.11* |
83.0 |
82.3 |
1.27 |
Organic matter |
83.3 |
84.2 |
2.05 |
84.6 |
82.9 |
2.02 |
NDF |
80.4 |
83.0 |
0.91** |
82.4 |
81.1 |
1.09 |
N |
78.6 |
82.8 |
2.27 |
82.1 |
79.3 |
2.40 |
* P<0.05; ** P<0.01 |
The voluntary feed intake was 44.7 and 45.6 g DM/kg live weight in
the Large White pigs of mean live weights 15.0 and 25.0 kg, respectively. It was
considered that consumption was ad libitum due to the fact
that some feed refusals were recorded. Average environmental temperature (August 2001) was
38 ± 3.1ºC at
Table
2. Comparison of methods of determination of digestibility indices in young Large
White pigs |
||||||
Method of estimation |
Live weight, kg |
|||||
Direct |
Indirect |
SEM |
15.0 |
25.0 |
SEM |
|
Digestibility,
% |
||||||
DM |
80.0 |
81.0 |
1.25 |
80.7 |
80.3 |
1.27 |
Organic matter |
83.3 |
82.9 |
1.08 |
83.9 |
82.2 |
1.10 |
NDF |
80.2 |
79.9 |
1.07 |
80.2 |
79.6 |
1.45 |
N |
84.6 |
84.1 |
1.12 |
85.0 |
83.7 |
1.08 |
Although not
strictly comparable, there was a consistent difference between both genotypes tested from
the point of view of voluntary feed intake, with the Mong Cai pigs having lower values compared
with the Large White animals. Experimental conditions were fairly similar with feeds based
predominantly on wheat bran and a high environmental temperature. In this context, it is
considered that a high feed intake negatively influences total tract digestibility indices
in pigs, especially when the feeding regime consists of feeds rich in cell walls (see for
example, Cunningham et al 1962; Roth and Kirchgessner 1984). A
similar negative effect on pig digestibility indices has been indicated for high
environmental temperature (Oude et al 1986; Jorgensen et al
1996).
The
influence of breed on digestibility indices has been studied both in European pig
genotypes selected for lean meat (Wilmink 1979; Wenk and Morel 1985) and in those raised in tropical areas (Teixeira et al 1995: Le Thi Men 1999; Ly et al 2001; Nguyen Thi Thuy
and Ly 2002). In this connection, it appears there is a slight trend toward a better
digestion of cell walls and a decrease in the digestion of N in Mong
Cai pigs, compared to improved Large White animals (Nguyen Thi
Thuy and Ly 2002; this study).
For the precise evaluation of feeds, it is recommended that both
age and weight of pigs from improved breeds should be considered (Lloyd et al 1957; Bayley and Summers 1975; Jorgensen et al 1978). However, in the
present investigation, the weight difference between the two groups of animals in each
experiment was relatively small, and this could be the cause of the absence of any
significant effect due to live weight.
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